Purification and Characterization of Proline Iminopeptidase from Lactobacillus casei ssp. casei LLG
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چکیده
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Proline iminopeptidase PepI overexpressing Lactobacillus casei as an adjunct starter in Edam cheese
In this study the growth of genetically modified Lactobacillus casei LAB6, overexpressing proline iminopeptidase PepI and its capacity to increase free proline was investigated during ripening of Edam cheese. The strain successfully survived 12 weeks of ripening period in cheese. The food-grade plasmid pLEB604, carrying the pepI gene, was stable, and PepI enzyme was active in LAB6 cells isolate...
متن کاملAntioxidative and hypocholesterolemic effect of Lactobacillus casei ssp casei (biodefensive properties of lactobacilli).
BACKGROUND A positive correlation between an individual's cholesterol level and development of CHD has been suggested. Low levels of high-density lipoprotein cholesterol (HDL-C) and high levels of low-density lipoprotein cholesterol (LDL-C) are important risk factors and oxidation of LDL has been implicated as an initiator of atherosclerosis. AIM Attempts are being made worldwide for the sear...
متن کاملProline iminopeptidase from Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397: purification and characterization.
Proline iminopeptidase (PepIP) is a major peptidase in Lactobacillus delbrueckii subsp. bularicus CNRZ397, encoded by the pepIP gene. Amplification and expression of this gene in Escherichia coli K12 resulted in a very high level of enzyme production. Moreover, export into the E. coli periplasm of 45% of PepIP activity allowed us to purify the enzyme easily by a single ion-exchange chromatograp...
متن کاملlactobacilli, Lactobacillus, Lactobacillus casei DN-114 001, Lactobacillus casei shirota, Lactobacillus coryniformis CECT5711, Lactobacillus gasseri CECT5714, Lactobacillus johnsonii LA1, Lactobacillus rhamnosus GR-1,Lactobacillus paracasei ssp. paracasei (CRL-
boulardii) Natural Standard Bottom Line Monograph, Copyright © 2012 (www.naturalstandard.com). Commercial distribution prohibited. This monograph is intended for informational purposes only, and should not be interpreted as specific medical advice. You should consult with a qualified healthcare provider before making decisions about therapies and/or health conditions. While some complementary a...
متن کاملdebittering of tryptic digests from β-casein and enzyme modified cheese by x-prolyl dipeptidylpeptidase from lactobacillus casei ssp. casei. llg
the proline-rich β-casein was digested in vitro with trypsin, and the oligopeptides producedwere then isolated by rp-hplc and subsequently identified by amino acid analysis and ion massspectrometry. the peptide fractions from the complete digestion were then treated with purified x-prolyldipeptidyl peptidase (x-pdp) extracted from lactobacillus casei ssp. casei llg. two bitter peptides (f53-97a...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1995
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(95)76632-2